Epicypher热销产品——Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated
[1] Lowary and Widom J. Mol. Biol. (1998). PMID: 9514715
产品详情
标签: Biotinylated
保存温度:Stable for six months at -80°C from date of receipt. For best results, aliquot and avoid multiple freeze/thaws.
产品形式: H2BK120ub dNuc (14.4 µg protein weight, 25 µg DNA + protein) in 22.5 µL 10 mM Tris HCl pH 7.5, 25 mM NaCl, 1 mM EDTA, 2 mM DTT, 20% glycerol. Molarity = 5.12 μM. MW = 216,985.9 Da.
验证数据
| Figure 1: Western blot data Western Analysis of Nucleosome, Recombinant Human, H2BK120ub. Top Panel: Unmodified H3 (Lane 1) and H2BK120ub containing nucleosomes (Lane 2) were probed with an anti-H2BK120ub antibody and analyzed via ECL readout. Only the H2BK120ub sample produced a detectable signal. Bottom Panel: Detail from Coomassie stained gel showing unmodified nucleosomes (Lane 1) and H2BK120ub nucleosomes (Lane 2). |
| Figure 2: Mass spec data H2BK120ub protein analyzed by high resolution mass spectrometry. Expected mass = 22,321.8 Da. Determined mass = 22,320.3 Da. |
| Figure 3: Protein gel data Coomassie stained PAGE gel of proteins in Nucleosome, Recombinant Human, H2BK120ub (1 μg) to demonstrate the purity of the histones in the preparation. Sizes of molecular weight markers and positions of the core histones (H2A, H2BK120ub, H3.1, and H4) are indicated. |
| Figure 4: DNA gel data Nucleosome, Recombinant Human, H2BK120ub dNucs resolved via native PAGE and stained with ethidium bromide to visualize DNA. Lane 1: Free DNA (100 ng). Lane 2: Intact H2BK120ub nucleosome (400 ng). |
订购详情
货号 | 产品名称 | 规格 |
16-0396 | Nucleosome, Recombinant Human, H2BK120ub1 dNuc, Biotinylated | 25 µg |
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