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Epicypher新品 | 核酸提取缓冲液

发布日期:2024-09-18
浏览次数:18

CUTANA™ 细胞核提取缓冲液是CUT&RUN和CUT&Tag实验中,用于从培养的细胞和组织中提取细胞核的必需试剂。这种缓冲液可与多种真核细胞和组织兼容。以下是使用该缓冲液成功进行细胞核分离的细胞和组织类型:


mouse NIH3T3   fibroblast cells

human A549   non-small cell lung cancer (NSCLC) cells

human K562 leukemia   cells

human   NCI-H1299 non-small cell lung cancer (NSCLS cells)

human bone   marrow derived macrophages

human TIG-1   fetal lung cells

human   monocyte derived macrophages

human LoVo   colorectal cancer cells

human   MV-4-11 macrophage cells

human LNCaP   prostate carcinoma cells

human SUM149   triple negative breast cancer (TNBC) cells

human renal   primary cells

human   GM24385 B-lymphocyte (aka HG002) cells

human   peripheral blood mononuclear cells (PBMCs)

human MCF7   breast cancer cells

human intestinal   tissue

human   MDA-MB-231 breast cancer cells

human HEPM   embryonic cells

human   SK-MEL-2 melanoma cells


 

只需将试剂盒中的Pre-Nuclei Extraction Buffer和亚精胺与蛋白酶抑制剂混合即可完成该试剂的制备。CUTANA™ 细胞核提取缓冲液针对CUT&RUN和CUT&Tag实验流程进行了优化,从而获得高质量的细胞核,助力您的基因组图谱分析。


试剂盒组成

组分

货号

CUTANA™ Pre-Nuclei Extraction Buffer        

21-1026a     

1 M   Spermidine

21-1026b


保存条件

将Pre-Nuclei Extraction Buffer 4ºC储存, 1 M亚精胺-20ºC储存。

自收到之日起可稳定储存6个月。


验证数据

验证数据.png

Figure 1: Nuclei Extraction

Nuclei were extracted from 3 different cell types: K562, NIH3T3, and LNCaP using the CUTANA™ Nuclei Extraction Protocol for CUT&RUN and CUT&Tag. Top 3 panels show cells before extraction. Starting cells are viable (bright white and round). Bottom 3 panels show nuclei extracted using the CUTANA™ Nuclei Extraction Buffer as indicated by positive Trypan Blue staining.


订购详情

货号

产品名称

规格

21-1026

CUTANA™   Nuclei Extraction Buffer

100   Reactions

 


如需了解更多详细信息或相关产品,请联系EpiCypher中国授权代理商-欣博盛生物 

全国服务热线: 4006-800-892       邮箱: market@hnyazd.com 

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广州:020-87615159           上海: 021-34613729

代理品牌网站: www.nobiosescience.com 

自主品牌网站: www.neobiosescience.net

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