睾酮(Testosterone)又称睾丸素、睾丸酮、睾甾酮，是分泌到血液中的最重要的雄性激素之一，由孕烯醇酮合成，孕烯醇酮本身由胆固醇形成。睾酮由男性的睾丸或女性的卵巢分泌，肾上腺亦分泌少量睾酮，其具有维持肌肉强度及质量、维持骨质密度及强度、提神及提升体能等作用。在成人中，每天大约合成5毫克睾酮，并在血浆中循环，主要与蛋白质结合，包括特异性激素结合球蛋白(SHBG)和非特异性蛋白质如白蛋白。据报道，生物可利用的睾酮包括游离类固醇和白蛋白结合类固醇，它们约占总睾酮的35%。
ENZO Testosterone ELISA kit是一种比色竞争法酶免疫检测试剂盒，3小时内可以得到检测结果。在405nm处读取吸光度。
● 高灵敏度，最低可检测5.67pg/ml睾酮
● 高通量，3小时内可得到检测结果
● 批间一致性好，是纵向研究的理想选择
● 超越半定量Western Blot分析的全定量检测
产品信息
产品货号 | ADI-900-065/ ADI-901-065 |
产品名称 | Testosterone ELISA kit（睾酮ELISA试剂盒） |
规格 | 96 wells/ 5x96 wells |
灵敏度 | 5.67 pg/ml |
检测范围 | 7.81 - 2,000 pg/ml |
检测时间 | 3 hours |
样本种属 | Species independent |
交叉反应 | Testosterone (100%), 19-hydroxytestosterone (14.6%), Androstendione (7.20%), Dehydroepiandrosterone (0.72%), Estradiol (0.40%) and<0.001%: Dihydrotestosterone, Estriol, Aldosterone, Corticosterone, Cortisol, Cortisone, Estrone, Progesterone, Pregenolone |
样本类型 | culture supernatants, plasma, serum, and saliva from any species. Not suitable for human serum and plasma. For those samples use product ADI-900-176. Cited sample types include tissue and feces |
保存温度 | +4°C |
试剂盒组分 | GxM IgG Microtiter plate, Conjugate, Antibody, Assay buffer 3, Wash buffer concentrate, Standard, Steroid displacement reagent, pNpp Substrate, Stop solution |
部分产品引用文献
1. 3-Monochloropropane-1,2-diol causes spermatogenesis failure in male rats via Sertoli cell dysfunction but not testosterone reduction: H. Xing, et al.; Toxicol. Lett. (2022)
2. Androgen conspires with the CD8+ T cell exhaustion program and contributes to sex bias in cancer: H. Kwon, et al.; Sci. Immunol. 7, eabq2630 (2022)
3. Deficiency of Wnt10a causes female infertility via the β-catenin/Cyp19a1 pathway in mice: J.H. Zhang, et al.; Int. J. Med. Sci. 19, 701 (2022)
4. Exogenous testosterone administration decreases rates of ovarian follicle growth in domestic chickens: C.R. Dean, et al.; Gen. Comp. Endocrinol. 329, 114121 (2022)
5. Non-invasive assessment of fecal glucocorticoid, progesterone, and androgen metabolites and microbiome in free-ranging southern white rhinoceros (Ceratotherium simum simum) in South Africa: K.H. Kothmann, et al.; Gen. Comp. Endocrinol. 329, 114099 (2022)
6. Purple Corn Extract Improves Benign Prostatic Hyperplasia by Regulating Prostate Cell Proliferation and Apoptosis: H.J. Kim, et al.; J. Agric. Food Chem. 70, 5561 (2022)
7. Sex, Organ, and Breed Differences in the mRNA Expression of Drug Transporters in the Liver and Kidney of Pigs: M. Kojima, et al.; Biol. Pharm. Bull. 45, 508 (2022)
8. Social dominance and reproduction result in increased integration of oxidative state in males of an African cichlid fish: R.J. Fialkowski, et al.; Comp. Biochem. Physiol. A Mol. Integr. Physiol. 269, 111216 (2022)
9. Androgen receptor phosphorylated at Ser815: The expression and function in the prostate and tumor-derived cells: K. Yokobori, et al.; Biochem. Pharmacol. 194, 114794 (2021)
10. Androgen-Dependent Differences in the Amounts of CYP mRNAs in the Pig Kidney: M. Kojima, et al.; Biol. Pharm. Bull. 44, 1120 (2021)
11. Early nighttime testosterone peaks are correlated with GnRH-induced testosterone in a diurnal songbird: T. Greives, et al.; Gen. Comp. Endocrinol. 312, 113861 (2021)
12. Exercise Training Promotes Sex-Specific Adaptations in Mouse Inguinal White Adipose Tissue: P. Nigro, et al.; Diabetes 70, 1250 (2021)
13. Impact of prenatal arsenic exposure on the testes and epididymides of prepubertal rats: A.C.F. Souza, et al.; Chem. Biol. Interact. 333, 109314 (2021), Application(s): ELISA using rat serum
14. Improvement of testosterone deficiency by fermented Momordica charantia extracts in aging male rats: K.S. Lee, et al.; Food Sci. Biotechno. 30, 443 (2021), Application(s): Blood samples
15. Investigation of equine testis contribution to vitamin D bioactivation: M. Rouge, et al.; Domest. Anim. Endocrinol. 79, 106691 (2021), Application(s): ELISA using horse plasma
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