高灵敏ELISA试剂盒，3小时内可检测低至1.40 pg/ml 6-酮前列腺素F1α
6-酮-前列环素F1α（6-keto-PGF1α）是前列环素（PGI2）的稳定水解产物。由于前列环素在缓冲液中的半衰期很短（2-3分钟），通常通过测量尿液中的6-酮-前列环素F1α代谢物及其衍生物2,3-二酮-前列环素F1α来监测前列环素的产生。前列环素具有自分泌功能，包括抑制血小板聚集、抑制加压激素导致的血管收缩反应、血小板解聚等特性以及生殖功能。这些效应是由受体介导的cAMP的升高所引起的。前列腺素在动脉粥样硬化和保护心肌缺血方面有临床应用。
Enzo Life Sciences的6-酮-前列环素F1α比色法ELISA试剂盒，可在3小时内得到检测结果，在405nm处读取OD值。该试剂盒特异性高，与结构相关的分子交叉反应性低。6-酮-前列环素F1α ELISA试剂盒已经在各种类型样品中得到了充分验证。欣博盛生物作为Enzo life sciences授权中国一级代理，可提供该产品，欢迎咨询。
产品特点
◆ 三小时内可快速得到38份样品（带复孔）的实验结果
◆ 高特异性，与结构相关分子交叉反应性低
◆ 已使用各种样品进行了充分验证，可靠性高
◆ 广泛的文献引用
产品信息
| 产品货号 | ADI-900-004/ ADI-901-004 |
产品名称 | 6-keto-PGF1α ELISA kit（6-酮前列腺素F1α ELISA试剂盒） |
规格 | 96 wells/ 5x96 wells |
其他名称 | 6-keto Prostaglandin F1α |
灵敏度 | 1.40 pg/ml |
检测范围 | 3.2 - 50,000 pg/ml |
应用 | ELISA, Colorimetric detection |
适用样本类型 | 细胞培养上清，血清，唾液和尿液 |
检测波长 | 405 nm |
适用种属 | 不限种属 |
运输 | Blue Ice Not Frozen |
长期保存 | +4°C |
试剂盒组分 | DxS IgG Microtiter plate, Conjugate, Antibody, Assay buffer, Wash buffer concentrate, Standard, pNpp Substrate, Stop solution |
标准曲线示例
部分产品引用文献
1. Laser Speckle Contrast Imaging-based diagnosis of severe mesenteric traction syndrome: Hemodynamics and prostacyclin - A prospective cohort study: A.A. Olsen, et al.; Microvasc. Res. 147, 104505 (2023)
2. Contribution of cyclooxygenase-1-dependent prostacyclin synthesis to bradykinin-induced dermal extravasation: M. Krybus, et al.; Biomed. Pharmacother. 148, 112786 (2022)
3. Differential effects of cyclo-oxygenase 1 and 2 inhibition on angiogenesis inhibitor-induced hypertension and kidney damage: K.M. Mirabito Colafella, et al.; Clin. Sci. (Lond.) 136, 675 (2022)
4. Estrogen modulates metabolic risk profile after resistance training in early postmenopausal women: a randomized controlled trial: T.V. Dam, et al.; Menopause 28, 1214 (2021)
5. Aspirin blocks formation of metastatic intravascular niches by inhibiting platelet-derived COX-1/thromboxane A2: S. Lucotti, et al.; J. Clin. Invest. 129, 1845 (2019)
6. Evidence for increased content of PGF2α, PGE2 and 6-keto-PGF1α in endometrial tissue cultures from heavy draft mares in anestrus with endometritis: M.J. Siemieniuch, et al.; J. Equine Vet. Sci. 77, 107 (2019)
7. Alterations in NO- and PGI2- dependent function in aorta in the orthotopic murine model of metastatic 4T1 breast cancer: relationship with pulmonary endothelial dysfunction and systemic inflammation: E. Buczek, et al.; BMC Cancer 18, 582 (2018)
8. Inhibitory Effects of an Orally Active Thromboxane A2 Receptor Antagonist, nstpbp5185, on Atherosclerosis in ApoE-Deficient Mice: S.W. Huang, et al.; Thromb. Haemost. 118, 401 (2018)
9. Advanced age in mares affects endometrial secretion of arachidonic acid metabolites during equine subclinical endometritis: M.J. Siemieniuch, et al.; Theriogenology 103, 191 (2017), Application(s): ELISA using culture supernatants
10. Colon cancer-derived myofibroblasts increase endothelial cell migration by glucocorticoid-sensitive secretion of a pro-migratory factor: Z. Drebert, et al.; Vascul. Pharmacol. 89, 19 (2017), Application(s): Determine the concentrations of prostanoids in conditioned medium from myofibroblasts and HUVECs, and in HUVEC lysates
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