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    BioXcell热销产品--BP0273 InVivoPlus anti-mouse PD-1 (CD279)

    2023-04-27
    浏览次数: 34

    BioXcell热销产品--BP0273  InVivoPlus anti-mouse PD-1 (CD279)

     

    产品描述:

    BioXcell InVivoPlus anti-mouse PD-1 29F.1A12单克隆抗体与小鼠PD-1(也称为CD279)反应。PD-1是一种50-55kDa的细胞表面受体,由Pdcd1基因编码,属于Ig超家族的CD28家族。PD-1在CD4和CD8胸腺细胞以及活化的T和B淋巴细胞和髓细胞上瞬时表达,在成功消除抗原后PD-1的表达下降。此外,在B细胞前阶段,Pdcd1 mRNA在发育中的B淋巴细胞中表达。PD-1的结构包括ITIM(基于免疫受体酪氨酸的抑制基序),这表明PD-1负调控TCR信号。PD-1通过结合B7家族的成员PD-L1和PD-L2发出信号。在配体结合后,PD-1信号传导抑制T细胞活化,导致增殖减少,细胞因子产生和T细胞死亡。此外,PD-1敲除动物表现出扩张型心肌病、脾肿大和外周耐受丧失,在小鼠的外周耐受性和预防自身免疫性疾病中发挥关键作用。诱导的PD-L1表达常见于许多肿瘤,包括鳞状细胞癌、结肠癌和乳腺癌。PD-L1过度表达会导致肿瘤细胞对CD8T细胞介导的裂解的抗性增加。在黑素瘤的小鼠模型中,可以通过用阻断PD-L1与其受体PD-1之间相互作用的抗体治疗来暂时抑制肿瘤生长。由于这些原因,目前正在探索抗PD-1介导的免疫疗法作为癌症治疗。与RMP1-14和J43抗体一样,BioXcell InVivoPlus anti-mouse PD-1 29F.1A12抗体在体内可阻断PD-1与其配体的结合。


    BioXcell热销产品--BP0273  InVivoPlus anti-mouse PD-1 (CD279) 


    产品详情:

    产品名称

    InVivoPlus anti-mouse PD-1 (CD279)

    产品货号

    BP0273

    产品规格

    5/25/50/100mg

    反应种属

    Mouse

    克隆号

    29F.1A12

    同种型

    Rat IgG2a

    免疫原

    Recombinant PD-1-Ig fusion protein

    实验应用

    in vivo blocking of PD-1/PD-L signaling

    in vitro PD-1 neutralization

    Immunohistochemistry (frozen)

    Immunofluorescence

    Western blot

    Flow cytometry

    产品形式

    PBS, pH 7.0,Contains no stabilizers or preservatives

    纯度

    >95%, Determined by SDS-PAGE

    聚合

    <5%, Determined by SEC

    无菌处理

    0.2 µm filtration

    纯化方式

    Protein G

    RRID

    AB_2687796

    分子量

    150 kDa

    小鼠病原检测

    Ectromelia/Mousepox Virus: Negative

    Hantavirus: Negative

    K Virus: Negative

    Lactate Dehydrogenase-Elevating Virus: Negative

    Lymphocytic Choriomeningitis virus: Negative

    Mouse Adenovirus: Negative

    Mouse Cytomegalovirus: Negative

    Mouse Hepatitis Virus: Negative

    Mouse Minute Virus: Negative

    Mouse Norovirus: Negative

    Mouse Parvovirus: Negative

    Mouse Rotavirus: Negative

    Mycoplasma Pulmonis: Negative

    Pneumonia Virus of Mice: Negative

    Polyoma Virus: Negative

    Reovirus Screen: Negative

    Sendai Virus: Negative

    Theiler’s Murine Encephalomyelitis: Negative

    保存条件

    抗体原液保存在4°C,不能冷冻保存。

    推荐同型对照

    InVivoPlus rat IgG2a isotype control, anti-trinitrophenol(货号BP0089)

    推荐抗体稀释液

    InVivoPure pH 7.0 Dilution Buffer(货号IP0070)

     

    为什么选择InVivoPlus抗体?

    InVivoPlus级别的产品内毒素含量更低,经过多种实验验证,更适合用于体内实验研究

     BioXcell热销产品--BP0273  InVivoPlus anti-mouse PD-1 (CD279)


     该产品自上市已被多篇SCI文献引用,品质有保证,以下是部分已发表的文献引用:


    应用

    文章

    体内PD-1/PD-L信号阻断

    (in vivo blocking of PD-1/PD-L signaling)

    1. Wang, W., et al. (2018). 'RIP1 Kinase Drives Macrophage-Mediated Adaptive Immune Tolerance in Pancreatic Cancer' Cancer Cell 34(5): 757-774 e757.

    2. Gordon, S. R., et al. (2017). 'PD-1 expression by tumour-associated macrophages inhibits phagocytosis and tumour immunity' Nature 545(7655): 495-499.

    3. Koyama, S., et al. (2016). 'STK11/LKB1 Deficiency Promotes Neutrophil Recruitment and Proinflammatory Cytokine Production to Suppress T-cell Activity in the Lung Tumor Microenvironment' Cancer Res 76(5): 999-1008.

    体内PD-1/PD-L信号阻断,流式细胞术

    (in vivo blocking of PD-1/PD-L signaling, Flow Cytometry)

    1.Koyama, S., et al. (2016). 'Adaptive resistance to therapeutic PD-1 blockade is associated with upregulation of alternative immune checkpoints' Nat Commun 7: 10501.

    体外PD-1中和

    (in vitro PD-1 neutralization)

    1.Park, S. J., et al. (2014). 'Negative role of inducible PD-1 on survival of activated dendritic cells' J Leukoc Biol 95(4): 621-629.

    体内PD-1/PD-L信号阻断,体外PD-1中和

    (in vivo blocking of PD-1/PD-L signaling, in vitro PD-1 neutralization)

    1.Duraiswamy, J., et al. (2013). 'Dual blockade of PD-1 and CTLA-4 combined with tumor vaccine effectively restores T-cell rejection function in tumors' Cancer Res 73(12): 3591-3603.

    流式细胞术

    (Flow Cytometry)

    1.Good-Jacobson, K. L., et al. (2012). 'CD80 expression on B cells regulates murine T follicular helper development, germinal center B cell survival, and plasma cell generation' J Immunol 188(9): 4217-4225.

     


    BioXcell热销产品--BP0273  InVivoPlus anti-mouse PD-1 (CD279)

    更多产品详情请咨询 BioXcell 中国授权代理——欣博盛生物

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