Enzo Life Sciences的ROS-ID®Hypoxia/Oxidative stress detection kit专门用于使用荧光显微镜或流式细胞术对活细胞（悬浮和贴壁）中的缺氧和氧化应激水平进行功能性检测。该试剂盒包含能够检测缺氧状态（红色）和氧化应激水平（绿色）的荧光探针。

检测缺氧的染料（红色）利用缺氧细胞中存在的硝基还原酶活性，将硝基基团转化为羟胺（NHOH）和氨基（NH2）并释放出荧光探针。

氧化应激检测试剂（绿色）是一种非荧光的、可透过细胞的总ROS检测染料，可与多种活性物质直接反应。可使用配备标准荧光素（490/525 nm）和德克萨斯红（596/670 nm）滤光片的宽场荧光显微镜、共聚焦显微镜或配备蓝色（488 nm）激光的流式细胞仪进行细胞分析。

产品特点

● 高灵敏度和特异性的荧光探针，可用于检测活细胞的缺氧和氧化应激

● 可用于分析贴壁或悬浮细胞系

● 试剂盒内包含整套试剂，包括ROS和缺氧诱导剂

实验示例

图1. 检测人HeLa和HL-60细胞中的缺氧和氧化应激水平。用缺氧诱导剂（DFO）和ROS诱导剂（pyocyanin）处理细胞。每个象限的数字反映了细胞（群体）的百分比。结果表明，缺氧和氧化应激染料具有特异性。

图2.使用不同试剂诱导HeLa细胞发生缺氧和氧化应激反应。缺氧探针在缺氧条件下被细胞硝基还原酶转化后观察到红色荧光。

图3.氧化应激（A）和缺氧（B）检测染料的吸收峰和发射峰分别为504nm/524nm和580nm/595nm。这些染料可以用488nm的氩离子激光器激发，并在流式细胞仪上的FL1通道（氧化应激染料）和FL3通道（缺氧红染料）中检测。

产品信息

部分产品引用文献

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2. Hydrogel microcapsules containing engineered bacteria for sustained production and release of protein drugs: C. Han, et al.; Biomaterials 287, 121619 (2022)

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5. Intrinsic radical species scavenging activities of tea polyphenols nanoparticles block pyroptosis in endotoxin-induced sepsis: Y. Chen, et al.; ACS Nano 16, 2429 (2022)

6. Iodinated cyanine dye-based nanosystem for synergistic phototherapy and hypoxia-activated bioreductive therapy: Y. Dong, et al.; Drug Deliv. 29, 238 (2022)

7. Lipoprotein-biomimetic nanostructure enables tumor-targeted penetration delivery for enhanced photo-gene therapy towards glioma: R. Wang, et al.; Bioact. Mater. 13, 286 (2022)

8. Microenvironment-driven sequential ferroptosis, photodynamic therapy, and chemotherapy for targeted breast cancer therapy by a cancer-cell-membrane-coated nanoscale metal-organic framework: W.L. Pan ,et al.; Biomaterials 283, 121559 (2022)

9. Mitochondrial glutathione depletion nanoshuttles for oxygen-irrelevant free radicals generation: A cascaded hierarchical targeting and theranostic strategy against hypoxic tumor: B. Liang, et al.; ACS Appl. Mater. Interfaces 14, 13038 (2022)

10. Multifunctional Nanosnowflakes for T1-T2 Double-Contrast Enhanced MRI and PAI Guided Oxygen Self-Supplementing Effective Anti-Tumor Therapy: Y. Lv, et al.; Int. J. Nanomedicine 17, 4619 (2022)

11. Physiologic flow-conditioning limits vascular dysfunction in engineered human capillaries: K. Haase, et al.; Biomaterials 280, 121248 (2022)

12. Platinum prodrug nanoparticles inhibiting tumor recurrence and metastasis by concurrent chemoradiotherapy: W. Jiang, et al.; J. Nanobiotechnology 20, 129 (2022)

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14. A cyclic nano-reactor achieving enhanced photodynamic tumor therapy by reversing multiple resistances: P. Liu, et al.; J. Nanobiotechnology 19, 149 (2021)

15. An albumin-based therapeutic nanosystem for photosensitizer/protein co-delivery to realize synergistic cancer therapy: S.L. Ai, et al.; ACS Appl. Bio. Mater. 4, 4946 (2021)

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