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天然免疫学热点领域科研抗体(AbD Serotec®)---巨噬细胞标志物抗体

2021-05-08
浏览次数: 2725
AbD Serotec公司是全球领先的巨噬细胞标志物抗体供应商,其生产的MCA497, Rat Anti Mouse F4/80 Clone CI:A3-1是著名的成熟小鼠巨噬细胞表面特异性标志物抗体,有将近400篇的文献引用,提供多达30种的包装形式,可用于FC、WB,IF、IHC等7种应用。AbD Serotec享有该克隆的专利,是该抗体唯一的商业化生产商。
MCA1957识别小鼠细胞表面macrosialin,为人CD68的同源物,是专门表达于组织巨噬细胞的一种重度糖基化的跨膜糖蛋白。

 天然免疫学热点领域科研抗体(AbD Serotec®)---巨噬细胞标志物抗体

 

小鼠淋巴结的免疫荧光染色;巨噬细胞用anti        小鼠脾脏冰冻切片的免疫组化染色;红髓中的
F4/80(MCA497PE)染成红色,B细胞用anti C-      巨噬细胞用anti CD68(MCA1957)染色
D79b(MCA1821A488)染成绿色,细胞核用D-
API复染,呈蓝色

AbD Serotec公司提供的最受欢迎的巨噬细胞标志物抗体
 人巨噬细胞标志物抗体
 产品名称
 克隆号
 产品编号
 形式
 CD11b
 ICRF44 
 MCA551
 Pur., A647, A700, Az. Fr., Biot., FITC, RPE, RPE-A647
 CD14 
 UCHM1
 MCA596
 Pur., A647, APC, Az. Fr., Biot., FITC, RPE
 CD14 
 TUK4 
 MCA1568 
 Pur., A647, A700, Biot., FITC, LE, PB, RPE, RPE-A647,  RPE-A750
 CD14 
 MEM-18
 MCA2185
 Pur., FITC, RPE
 CD14 
 61D3
 MCA2804
 RPE-Cy5, RPE-Cy5.5
 CD163
 EDHu-1 
 MCA1853 
 Pur., FITC
 CD169 
 7-239 
 MCA2517
 Pur., A488, A647, FITC, LE, RPE
 CD200R 
 OX108 
 MCA2282 
 Pur., A488, A647, Biot., FITC, RPE
 CD204 
 Poly 
 AHP563 
 Serum
 CD206 
 15-2 
 MCA2155 
 Pur.
 CD209 
 MR-1
 MCA2318 
 Pur., A488, A647, Biot., FITC, LE, RPE
 CD31 
 WM59 
 MCA1738
 Pur., A647, Biot., FITC, RPE
 CD36 
 SMØ 
 MCA722 
 Pur., A647, Az. Fr., FITC
 CD68 
 514H12
 MCA1815 
 S/N
 CD68 
 KiM7
 MCA2375F
 FITC
 Dectin-1 
 BD6
 MCA4662
 Pur., A488, A647, FITC, RPE
 Dectin-1 
 GE2
 MCA4661 
 Pur., A488, A647, FITC, LE, RPE
 Macrophage 
 MAC387 
 MCA874 
 Pur., A488, FITC, LE, RPE
 小鼠巨噬细胞标志物抗体
 CD11b
 M1/70.15
 MCA74
 Pur., A488, A647, A700, Biot., FITC, LE, RPE, RPE-
 A647, RPE-A750, RPE-Cy5
 CD11b
 5C6
 MCA711
 Pur., FITC, LE, RPE
 CD115
 604B5
 2E11
 MCA1898 Pur., Az. Fr., RPE
 CD169
 MOMA-1
 MCA947
 Pur., S/N, A647, FITC
 CD169
 3D6.112
 MCA884
 Pur., FITC, LE
 CD200R
 OX-110
 MCA2281
 Pur., A488, A647, Biot., FITC, LE, RPE
 CD204
 2F8
 MCA1322
 Pur., A488, A647, Biot., FITC, LE, RPE
 CD206
 MR5D3
 MCA2235
 Pur., A488, A647, Biot., FITC, LE, RPE
 CD31
 ER-MP12
 MCA2388
 Pur., A488, A647, Biot., RPE, RPE-A647
 CD36
 MF3
 MCA2748
 Pur., A488, A647, FITC, LE, RPE
 CD68
 FA-11
 MCA1957
 Pur., A488, A647, A700, Az. Fr., Biot., FITC, RPE
 Dectin-1  
 2A11
 MCA2289
 Pur., A647, Biot., FITC, LE, RPE
 Dectin-2
 D2.11E4
 MCA2415
 Pur., A488, A647, Biot., FITC, LE, RPE
 F4/80
 CI:A3-1
 MCA497
 Pur., A488, A647, A700, APC, Biot., FITC, LE, PB, RPE,    RPE-A647, RPE-A750, S/N
 Ly-6B.2
 7/4
 MCA771
 Pur., A647, A700, Biot., FITC, LE, RPE
 Ly-6C
 ER-MP20
 MCA2389
 Pur., A488, A647, Biot., RPE
 MARCO
 ED31
 MCA1849
 Pur., FITC, RPE
 TREM-1
 L5-B8.2A12.3A12
 MCA2578
 Pur., A488, A647, FITC, LE, RPE
 TREM-2
  78.18
 MCA4772
 Pur., FITC, LE
 大鼠巨噬细胞标志物抗体
 CD11b
 OX-42
 MCA275
 Pur., A488, A647, A700, Biot., FITC, LE, PB, RPE, RPE-
 A647
 CD11b
 ED7
 MCA618R
 Pur.
 CD11b
 ED8
 MCA619
 Pur., FITC, RPE
 CD115
 604B52E11
 MCA1898
 Pur., Az. Fr., RPE
 CD163
 ED2
 MCA342
 Pur., A647, Biot., FITC, LE, RPE
 CD169
 ED3
 MCA343
 Pur.
 CD172a
 OX41
 MCA274
 Pur., Az. Fr., Biot, FITC, RPE
 CD172a
 ED9
 MCA620
 Pur., FITC, RPE
 CD200R1
 OX-102
 MCA1959
 Pur., Biot., FITC, LE, RPE
 CD31
TLD-3A12
 MCA1334
 Pur., A488, A647, Biot., FITC, LE, RPE
 CD68
 ED1
 MCA341
 Pur., A488, A647, A700, Biot., FITC, RPE


荧光多色染色试剂
 产品名称
 克隆号
 产品编号
 形式
 Human CD45/CD14
 F10-89-4/UCHM1
 DC008
 FITC/RPE
 Mouse IgG2a/IgG2a 
 Negative Control
 DC013
  FITC/RPE
 Mouse Ly6C/CD31
 ERMP20/ERMP12
 DC053
 A488/RPE
 Mouse Ly6C/CD31
 ERMP20/ERMP12
 DC054
 A488/RPE-A647
 Mouse CD11b/CD115
 M1/70.15 / 604B5 2E11
 DC055
 A488/RPE
 Mouse Ly6C/CD115/CD11b
 ERMP20/604B52E11/M1/70.15
 TC015
 A488/RPE/RPE-A647
 Mouse Ly6C/CD115/CD31
 ERMP20/604B52E11/ERMP12
 TC016
 A488/RPE/RPE-A647
 Mouse Ly6C/CD115/CD31
 ERMP20/604B52E11/ERMP12
 TC017
 A488/RPE/A647
 Mouse Ly6C/CD115/CD11b/CD31
 ERMP20/604B52E11/M1/70.15/ERMP12
 QC001
 A488/RPE/RPE-A647/A647


AbD Serotec公司 F4/80 产品参考文献
1. Austyn, J.M. and Gordon, S. (1981) F4/80 , a monoclonal antibody directed specifically against the mouse macrophage.Eur. J. Immunol. 11: 805-815.
2. Hume, D.A., Perry, V.H., and Gordon, S. (1984) The Mononuclear Phagocyte System of the Mouse defined by Immunohistochemical Localisation of Antigen F4/80: Macrophages Associated with Epithelia.The Anatomical Record 210: 503-512.
3. Lee, S.H. et al. (1985) Quantitative analysis of total macrophage content in adult mouse tissues. Immunochemical studies with monoclonal antibody F4/80.J. Exp. Med. 161: 475-489.
4. Gordon, S. et al. (1992) Antigen markers of macrophage differentiation in murine tissues.
Curr. Top. Micro. Immunol. 181: 1-37.
5. Uaesoontrachoon, K. et al. (2013) Osteopontin deficiency delays inflammatory infiltration and the onset of muscle regeneration in a mouse model of muscle injury.Dis Model Mech. 6: 197-205.
6. Lin, H. H.et al. (2005) The macrophage F4/80 receptor is required for the induction of antigen-specific efferent regulatory T cells in peripheral tolerance.J. Exp. Med. 201: 1615–1625.
7. Chan, R.J. et al. (2005) Human somatic PTPN11 mutations induce hematopoietic cell hypersensitivity to granulocyte-macrophage colony stimulating factor.Blood. 105: 3737-3742.
8. Pizza, F.X. et al. (2005) Neutrophils contribute to muscle injury and impair its resolution after lengthening contractions in mice.J. Physiol. 562:899-913.
9. Tarallo, V. et al. (2011) The biflavonoid amentoflavone inhibits neovascularization preventing the activity of proangiogenic vascular endothelial growth factors.J Biol Chem. 286: 19641-51.
10. Rivollier, A. et al. (2012) Inflammation switches the differentiation program of Ly6Chi monocytes from antiinflammatory macrophages to inflammatory dendritic cells in the colon.
J Exp Med. 209: 139-55.
11. Hemmi, H. et al. (2009) A new triggering receptor expressed on myeloid cells (Trem) family member,Trem-like 4, binds to dead cells and is a DNAX activation protein 12-linkedmarker for subsets of mouse macrophages and dendritic cells.J Immunol. 182:1278-86.
12. Seitz, O. et al. (2010) Wound Healing in Mice with High-Fat Diet- or ob Gene-Induced Diabetes-Obesity Syndromes: A Comparative Study.Exp Diabetes Res. 2010: 476969. 
13. Miao, E.A. et al. (2011) Caspase-1-induced pyroptosis is an innate immune effector mechanism against intracellular bacteria.Nat Immunol. 11: 1136-42.
14. Wang, X. et al. (2011) Activation of the cholinergic antiinflammatory pathway ameliorates obesity-induced inflammation and insulin resistance.Endocrinology. 152: 836-46.
15. Cunningham, O. et al. (2009) Microglia and the urokinase plasminogen activator receptor/uPA system ininnate brain inflammation.Glia. 57: 1802-14.
16. Gornicka, A. et al. (2012) Adipocyte hypertrophy is associated with lysosomal permeability both in vivo and in vitro: role in adipose tissue inflammation.Am J Physiol Endocrinol Metab. 303: E597-606.
17. Akbarshahi, H. et al. (2012) Enrichment of Murine CD68(+)CCR2(+) and CD68(+)CD206(+) Lung Macrophages in Acute Pancreatitis-Associated Acute Lung Injury.PLoS One. 7: e42654.
18. Banda, N.K. et al. (1012) Role of C3a receptors, C5a receptors, and complement protein C6 deficiency in collagen antibody-induced arthritis in mice.J Immunol. 188: 1469-78. 
19. Bonde, A.K. et al. (2012) Intratumoral macrophages contribute to epithelial-mesenchymal transition in solid tumors.BMC Cancer. 12: 35.
20. Choi, K.M. et al. (2010) CD206-positive M2 macrophages that express heme oxygenase-1 protect against diabetic gastroparesis in mice.Gastroenterology. 138: 2399-409, 
21. Tamaki, S. et al. (2013) Interleukin-16 promotes cardiac fibrosis and myocardial stiffening in heart failure with preserved ejection fraction.PLoS One. 8: e68893.
22. Kihira, Y. et al. (2014) Deletion of hypoxia-inducible factor-1a in adipocytes enhances glucagon-like Peptide-1 secretion and reduces adipose tissue inflammation.
PLoS One. 9(4):e93856.


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