c-Kit/CD117 GNNK− Isoform Extracellular Domain (human, recombinant)

c-Kit Isoform 2|c-Kit(−)|Cluster of Differentiation 117|Mast/Stem Cell Growth Factor Receptor Kit|Proto-oncogene c-Kit|SCFR|v-Kit Hardy-Zuckerman 4 Feline Sarcoma Viral Oncogene Homolog

c-Kit, also known as CD117, is a type III receptor tyrosine kinase involved in the proliferation, differentiation, and survival of hematopoietic cells.{58189,54662} It is composed of an N-terminal extracellular domain (ECD) containing five immunoglobulin-like (Ig-like) domains, short transmembrane and juxtamembrane domains, and a C-terminal intracellular region containing the kinase domain. Alternative splicing of KIT produces two isoforms, GNNK+ and GNNK−, that contain or do not contain the tetrapeptide GNNK, respectively, in the juxtamembrane region of the ECD.{68236} c-Kit is expressed in hematopoietic stem and progenitor cells, as well as certain non-hematopoietic tissues.{54662} It is activated by the binding of its ligand, stem cell factor (SCF), to the extracellular region, which induces autophosphorylation and the recruitment of signaling molecules to the intracellular region.{58189} c-Kit signal transduction activates a variety of signaling pathways, including the PI3K/Akt, RAS/MAPK, and JAK/STAT pathways.{58189,54662} The GNNK− isoform of c-Kit undergoes more rapid autophosphorylation and internalization and shows increased MAPK signaling compared with GNNK+ c-Kit, promotes anchorage-independent growth in vitro, and induces tumorigenesis in mice.{68236} Homozygous knockout of Kit, the gene encoding c-Kit, is embryonic lethal, with mice developing severe anemia in utero.{54663} KIT is considered a proto-oncogene and activating mutations have been associated with a variety of cancers.{58189,54662} Cayman’s c-Kit/CD117 GNNK− Isoform Extracellular Domain (human, recombinant) protein consists of 502 amino acids, has a calculated molecular weight of 56.7 kDa, and a predicted N-terminus of Gln26 after signal peptide cleavage. By SDS-PAGE, under reducing conditions, the apparent molecular mass of the protein is 86 kDa due to glycosylation.

HEK293 cells

-80

-80

Lyophilized from sterile PBS, pH 7.4

365

≥97% as determined by SDS-PAGE