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折扣/货期| 货号：GTX01501 | 规格：1mg | 目录价：￥1200 |
产品详情
产品名称:
WGA Lectin (Biotin)
别名:
Wheat Germ agglutinin , Triticum vulgaris , Wheat germ agglutinin lectin , Wheat germ agglutinin , Triticum vulgaris , GlcNAc lectin , WGA , WGA Lectin , Triticum vulgaris lectin
产品描述:
Wheat germ agglutinin (WGA) (MW 36 kDa) is a dimeric, carbohydrate-free protein composed of two identical subunits. The amino acid sequence provides a figure of 21,600, based on 171 amino acids per polypeptide chain. The protein dissociates into monomers in acidic media, pK of the reaction 4. The receptor sugar for WGA is N-acetyl glucosamine (GlcNAc), GlcNacβ14GlcNAc. These structures are present in many serum and membrane glycoproteins, bacterial cell wall peptidoglycans, chitin, cartilage glycosaminogyycons and glycolipids. Native WGA also react with glycoproteins with sialic acid residue. This lectin is useful for the purification of insulin receptors, serum proteins and neuronal tracing. The carbohydrate-binding specificity of WGA has been studied by variety of techniques, such as hapten inhibition of hemagglutination and specific precipitation of glycoconjugate, changes in fluorescence of the lectin (intrinsic) or of chromatogenic ligands (extrinsic), equilibrium dialysis, NMR and x-ray diffraction.
实验应用:
ELISA, IHC, WB
靶标/特异性:
N-acetyl glucosamine (GlcNAc)
克隆号:
偶联:
Biotin
产品浓度:
5 mg/ml (Please refer to the vial label for the specific concentration.)
表达系统:
Native Protein
保存温度:
Store as concentrated solution. Centrifuge briefly prior to opening vial. Store at 4ºC.
运输温度:
4°C
产品形式:
Liquid
存储溶液:
10mM Phosphate, 150mM NaCl, 0.1mM CaCl₂, 0.05% Sodium azide.
产地:
美国
功能与背景:
Wheat germ agglutinin (WGA) (MW 36 kDa) is a dimeric, carbohydrate-free protein composed of two identical subunits. The amino acid sequence provides a figure of 21,600, based on 171 amino acids per polypeptide chain. The protein dissociates into monomers in acidic media, pK of the reaction 4. The receptor sugar for WGA is N-acetyl glucosamine (GlcNAc), GlcNacβ14GlcNAc. These structures are present in many serum and membrane glycoproteins, bacterial cell wall peptidoglycans, chitin, cartilage glycosaminogyycons and glycolipids. Native WGA also react with glycoproteins with sialic acid residue. This lectin is useful for the purification of insulin receptors, serum proteins and neuronal tracing. The carbohydrate-binding specificity of WGA has been studied by variety of techniques, such as hapten inhibition of hemagglutination and specific precipitation of glycoconjugate, changes in fluorescence of the lectin (intrinsic) or of chromatogenic ligands (extrinsic), equilibrium dialysis, NMR and x-ray diffraction. Inhibiting/Eluting sugars: 500 mM GlcNAc, dimer, trimmer or 100 mM acetic acid.Carbohydrate-Binding Specificity of WGA: Pentaacetylchitopentaose >Tetraacetylchitotetraose > triacetylchitotriosese > Diacetylchitobiosese > GlcNAc
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